Contact

Giuseppina Maccarrone, PhD
Giuseppina Maccarrone, PhD
Head of Core Unit
Phone: +49 (0) 89-30622-211 /-597

Instrumentation

<p><strong>Q-Tof Impact HD II</strong></p>
High resolution quadrupole time-of-flight (Q-Tof) tandem mass spectrometer, equipped with nano electrospray ion source and a nanoBooster to enhance the ion signal intensity. It is online coupled to a nano 2D-UHPLC, Dionex Ultimate 3000 RSLC. This LC-MS/MS system is used to perform high throughput identification and quantification of proteins in complex sample mixtures. Zoom Image

Q-Tof Impact HD II

High resolution quadrupole time-of-flight (Q-Tof) tandem mass spectrometer, equipped with nano electrospray ion source and a nanoBooster to enhance the ion signal intensity. It is online coupled to a nano 2D-UHPLC, Dionex Ultimate 3000 RSLC. This LC-MS/MS system is used to perform high throughput identification and quantification of proteins in complex sample mixtures. [less]
<strong>LTQ Orbitrap XL</strong> Tandem mass spectrometer equipped with nano electrospray ion source, online coupled to a nano 2D-HPLC. This LC-MS/MS system is capable of performing high throughput analyses of complex peptide mixtures with fmol sensitivity. Zoom Image
LTQ Orbitrap XL Tandem mass spectrometer equipped with nano electrospray ion source, online coupled to a nano 2D-HPLC. This LC-MS/MS system is capable of performing high throughput analyses of complex peptide mixtures with fmol sensitivity. [less]
<strong>HPLC </strong>Two HPLC systems equipped with thermostat autosampler, column oven, UV/Vis detector and thermostat fraction collector for 96 well microtiter plates. Pumps allow 0.1 to 20 &mu;l/min and 0.1 to 2.5 ml/min flow rates, respectively. Used for prefractionation of peptides and proteins. Zoom Image
HPLC Two HPLC systems equipped with thermostat autosampler, column oven, UV/Vis detector and thermostat fraction collector for 96 well microtiter plates. Pumps allow 0.1 to 20 μl/min and 0.1 to 2.5 ml/min flow rates, respectively. Used for prefractionation of peptides and proteins. [less]
<strong>Gel Scanners</strong>
<p>1D SDS-PAGE and 2D-PAGE gel image acquisition. In combination with Quantity One and PD Quest<sup>TM </sup>softwares used for relative protein quantification. Fluorescent scanner FX-imager / Densitometer GS-800.</p> Zoom Image
Gel Scanners

1D SDS-PAGE and 2D-PAGE gel image acquisition. In combination with Quantity One and PD QuestTM softwares used for relative protein quantification. Fluorescent scanner FX-imager / Densitometer GS-800.

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Proteomics

Proteomic analyses tend to be complex. Prior to sample submission, we recommend contacting us to discuss details on experimental design and sample preparation as well as data analysis strategies.

The Proteomics Core Unit provides consulting and state-of-the-art technologies for peptide and protein

  • identification
  • characterization
  • quantification

Analysis methods include

  • Peptide and recombinant protein molecular weight determination
  • Protein identification
  • Mapping of post-translational protein modifications
  • Multidimensional protein and peptide separation
  • Relative protein quantification

We are using the following methods and equipment

  • Nano LC-MS/MS (LTQ-Orbitrap)
  • Q-Tof Impact HD II
  • Micro/Capillary-High Performance Liquid Chromatography (HPLC)
  • Isoelectric Focusing (IEF)
  • One dimensional SDS-Polyacrylamide Gel Electrophoresis (1D SDS-PAGE)
  • Two-dimensional Polyacrylamide Gel Electrophoresis (2D-PAGE)

Samples are processed from a variety of species and include

  • Cellular extracts
  • Tissue extracts
  • Plasma
  • Immunoprecipitates

Sample preparation for mass spectrometry-based proteomic analysis

Zoom Image
  • Protein fractionation by 1D SDS-PAGE or 2D-PAGE followed by Coomassie staining and in-gel digestion
  • Peptide fractionation by IEF
  • Protein and peptide fractionation by micro- and capillary-liquid chromatography

Mass spectrometry analysis

Zoom Image
  • Molecular weight determination of synthetic peptides and recombinant proteins
  • Protein/peptide identification from in-gel digests
  • Multidimensional protein and peptide separations (2D-PAGE, IEF/LC, LC/LC)
  • Characterization and mapping of post-translational protein modifications (e.g., phosphorylation, ubiquitination, acetylation)
  • Relative protein quantification (label free, gel-based, chemical labeling, metabolic labeling)
  • Targeted protein/peptide quantification
 
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