A stress protein is essential for antidepressant action
Scientists discover an unexpected link between the well-known stress protein FKBP51, autophagy and the effectiveness of antidepressants
While depression is the most common psychiatric disease worldwide, only one third of the patients achieve sustained remission following treatment with currently available antidepressants. Scientists at the Max Planck Institute of Psychiatry in Munich have now unraveled a mechanism by which the stress-related protein FKBP51 affects antidepressant efficacy. In a combined interdisciplinary research approach working with cell cultures, mouse models and finally patient samples, the scientific team observed that FKBP51 acting together with antidepressants organizes intracellular complexes important for autophagy. This intracellular process of “self-digestion” ensures efficient recycling of damaged material and has recently been linked to a range of different diseases. The discoveries are important steps towards the development of novel antidepressant drugs and the implementation of personalized medicine.
The poor effectiveness of antidepressants is at least partially due to a lack in understanding of the mechanism of action of these drugs. The FK506 binding protein 51 (FKBP51) is a known regulator of stress-related receptors in the brain. It interacts with the so-called glucocorticoid receptor, thus influencing the stress hormone axis and stress physiology in general. Previous research at the Max Planck Institute of Psychiatry in Munich already provided a link between the genetic variants of FKBP51 and the efficacy of different antidepressants in patients.
Scientists around the group leaders Theo Rein and Mathias Schmidt have now unraveled the mechanism by which the protein FKBP51 affects antidepressant efficacy. The researchers found that FKBP51 orchestrates the composition and activity of protein complexes driving autophagy. Autophagy is an intracellular process of “self-digestion” that enables efficient recycling of damaged intracellular material, thereby ensuring cellular integrity and functioning. Autophagy has been established as a pivotal process in a range of (patho-) physiological conditions, including cancer, diabetes and more recently neurotransmission or neurodegeneration. In the context of depression, the molecular actions of FKBP51 on autophagy synergize with those of antidepressants. “In cultured cells that do not express FKBP51, antidepressants could not elicit autophagy,” explain Nils Gassen and Jakob Hartmann, shared first authors of the current study. “Thus, FKBP51 sets the stage for antidepressants to act on the brain.”
Using mouse models, the researchers observed that missing FKBP51 largely abolished the effects of antidepressants on behavior and on autophagic markers. For example, treatment with the antidepressant paroxetine enhanced social behavior in stressed wildtype mice but not in those with deletion of FKBP51. To evaluate the clinical values of their findings, the team investigated the effects of FKBP51 in human blood cells. Protein analysis confirmed the correlation between the expression levels of FKBP51 and the levels of autophagy markers and FKBP51 was required for the effects of antidepressants on autophagic pathways. Treating cultivated blood cells from patients at the time of admission with antidepressants allowed prediction of the therapeutic outcome for the patient after 6 weeks.
“Taken together, our finding of FKBP51-dependent antidepressants’ effects on intracellular pathways, brain function and behavior strengthens the relevance of the genetic association of FKBP51 with antidepressant response and furthermore substantiates the need for more targeted studies,” concludes Theo Rein. “Nevertheless, given the complexity of depression and the plethora of reported molecular effects of antidepressants, it is unlikely that all antidepressants, current or future, will depend on FKBP51 or any other single protein. Yet we suggest that autophagy-initiating mechanisms should be considered as a pharmacological target to improve the treatment of depression.”