Equipment Maccarrone

We are using the following methods and equipment

  • Nano LC-MS/MS (LTQ-Orbitrap)
  • Q-Tof Impact HD II
  • Micro/Capillary-High Performance Liquid Chromatography (HPLC)
  • Isoelectric Focusing (IEF)
  • One dimensional SDS-Polyacrylamide Gel Electrophoresis (1D SDS-PAGE)
  • Two-dimensional Polyacrylamide Gel Electrophoresis (2D-PAGE)

Samples are processed from a variety of species and include

  • Cellular extracts
  • Tissue extracts
  • Plasma
  • Immunoprecipitates

Instrumentation

Q-Tof Impact HD II High resolution quadrupole time-of-flight (Q-Tof) tandem mass spectrometer, equipped with nano electrospray ion source and a nanoBooster to enhance the ion signal intensity. It is online coupled to a nano 2D-UHPLC, Dionex Ultimate 3000 RSLC. This LC-MS/MS system is used to perform high throughput identification and quantification of proteins in complex sample mixtures.

LTQ Orbitrap XL Tandem mass spectrometer equipped with nano electrospray ion source, online coupled to a nano 2D-HPLC. This LC-MS/MS system is capable of performing high throughput analyses of complex peptide mixtures with fmol sensitivity.

HPLC Two HPLC systems equipped with thermostat autosampler, column oven, UV/Vis detector and thermostat fraction collector for 96 well microtiter plates. Pumps allow 0.1 to 20 μl/min and 0.1 to 2.5 ml/min flow rates, respectively. Used for prefractionation of peptides and proteins.

Gel Scanners 1D SDS-PAGE and 2D-PAGE gel image acquisition. In combination with Quantity One and PD QuestTM softwares used for relative protein quantification. Fluorescent scanner FX-imager / Densitometer GS-800.

Go to Editor View