Service Maccarrone

The Proteomics Core Unit provides consulting and state-of-the-art technologies for peptide and protein

  • identification
  • characterization
  • quantification

Analysis methods include

  • Peptide and recombinant protein molecular weight determination
  • Protein identification
  • Mapping of post-translational protein modifications
  • Multidimensional protein and peptide separation
  • Relative protein quantification

Sample preparation for mass spectrometry-based proteomic analysis

  • Protein fractionation by 1D SDS-PAGE or 2D-PAGE followed by Coomassie staining and in-gel digestion
  • Peptide fractionation by IEF
  • Protein and peptide fractionation by micro- and capillary-liquid chromatography

Mass spectrometry analysis

  • Molecular weight determination of synthetic peptides and recombinant proteins
  • Protein/peptide identification from in-gel digests
  • Multidimensional protein and peptide separations (2D-PAGE, IEF/LC, LC/LC)
  • Characterization and mapping of post-translational protein modifications (e.g., phosphorylation, ubiquitination, acetylation)
  • Relative protein quantification (label free, gel-based, chemical labeling, metabolic labeling)
  • Targeted protein/peptide quantification
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