Service Maccarrone
The Proteomics Core Unit provides consulting and state-of-the-art technologies for peptide and protein
- identification
- characterization
- quantification
Analysis methods include
- Peptide and recombinant protein molecular weight determination
- Protein identification
- Mapping of post-translational protein modifications
- Multidimensional protein and peptide separation
- Relative protein quantification
Sample preparation for mass spectrometry-based proteomic analysis
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- Protein fractionation by 1D SDS-PAGE or 2D-PAGE followed by Coomassie staining and in-gel digestion
- Peptide fractionation by IEF
- Protein and peptide fractionation by micro- and capillary-liquid chromatography
Mass spectrometry analysis
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- Molecular weight determination of synthetic peptides and recombinant proteins
- Protein/peptide identification from in-gel digests
- Multidimensional protein and peptide separations (2D-PAGE, IEF/LC, LC/LC)
- Characterization and mapping of post-translational protein modifications (e.g., phosphorylation, ubiquitination, acetylation)
- Relative protein quantification (label free, gel-based, chemical labeling, metabolic labeling)
- Targeted protein/peptide quantification